| Host: |
Rabbit |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MuRF1 (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:300IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRIM63 |
| Gene ID: |
84676 |
| Uniprot ID: |
TRI63_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
The antibody detects 38 and 30 kDa* proteins corresponding to the apparent molecular mass of MuRF1 isoforms on SDS-PAGE immunoblots of mouse C2C12 cells, and detects a 38 kDa band in mouse heart and muscle tissue. |
| Immunogen: |
MuRF1 (C-terminal) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55). |
| Tissue Specificity | Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris. |
| Function | E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim63Iris Ring Finger ProteinMuscle-Specific Ring Finger Protein 1Murf-1Murf1Ring Finger Protein 28Ring-Type E3 Ubiquitin Transferase Trim63Striated Muscle Ring Zinc Finger ProteinTripartite Motif-Containing Protein 63 |
| Database Links | Reactome: R-HSA-9615017Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusMyofibrilSarcomereM LineZ LineColocalizes With Tnni3 In MyocytesLocalizes To The M- And Z-Lines In Skeletal Muscle |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim63 antibodyAnti-Iris Ring Finger Protein antibodyAnti-Muscle-Specific Ring Finger Protein 1 antibodyAnti-Murf-1 antibodyAnti-Murf1 antibodyAnti-Ring Finger Protein 28 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim63 antibodyAnti-Striated Muscle Ring Zinc Finger Protein antibodyAnti-Tripartite Motif-Containing Protein 63 antibodyAnti-TRIM63 antibodyAnti-IRF antibodyAnti-MURF1 antibodyAnti-RNF28 antibodyAnti-SMRZ antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
