| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-E3 ubiquitin-protein ligase TRIM50 (312-362 aa) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRIM50 |
| Gene ID: | 135892 |
| Uniprot ID: | TRI50_HUMAN |
| Immunogen Region: | 312-362 aa |
| Specificity: | This antibody detects endogenous levels of TRI50 at Human/Mouse/Rat |
| Immunogen: | Synthesized peptide derived from the human TRI50 at the amino acid range 312-362 |
| Function | E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Also interacts with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction. Also promotes NLRP3 inflammasome activation by directly inducing NLRP3 oligomerization independent of its E3 ligase function. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim50Ring-Type E3 Ubiquitin Transferase Trim50Tripartite Motif-Containing Protein 50 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmLocalizes Mainly Into Discrete Cytoplasmic Punctuate Structures Heterogeneous In Size And Shape Containing Polyubiquitinated Proteins |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim50 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim50 antibodyAnti-Tripartite Motif-Containing Protein 50 antibodyAnti-TRIM50 antibodyAnti-TRIM50A antibody |
Information sourced from Uniprot.org
