Anti-TRIM5 antibody (100-200) (STJ117788)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TRIM5 (100-200) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM5 |
| Gene ID | 85363 |
| Uniprot ID | TRIM5_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | EKLLLFCQEDGKVICWLCER SQEHRGHHTFLTEEVAREYQ VKLQAALEMLRQKQQEAEEL EADIREEKASWKTQIQYDKT NVLADFEQLRDILDWEESNE L |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM5 (NP_149083.2). |
Additional Info
| Post Translational Modifications | Degraded in a proteasome-independent fashion in the absence of viral infection but in a proteasome-dependent fashion following exposure to restriction sensitive virus. Autoubiquitinated in a RING finger- and UBE2D2-dependent manner. Monoubiquitinated by TRIM21. Deubiquitinated by Yersinia YopJ. Ubiquitination may not lead to proteasomal degradation. |
| Function | Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2. Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction. |
| Protein Name | Tripartite Motif-Containing Protein 5Ring Finger Protein 88Ring-Type E3 Ubiquitin Transferase Trim5 |
| Database Links | Reactome: R-HSA-877300 |
| Cellular Localisation | CytoplasmNucleusPredominantly Localizes In Cytoplasmic BodiesLocalization May Be Influenced By The Coexpression Of Other Trim ProteinsHence Partial Nuclear Localization Is Observed In The Presence Of Trim22 Or Trim27In Cytoplasmic BodiesColocalizes With Proteasomal Subunits And Sqstm1 |
| Alternative Antibody Names | Anti-Tripartite Motif-Containing Protein 5 antibodyAnti-Ring Finger Protein 88 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim5 antibodyAnti-TRIM5 antibodyAnti-RNF88 antibody |
Information sourced from Uniprot.org