• Immunofluorescence analysis of L929 cells using TRIM47 antibody (STJ119831) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of Mouse stomach, using TRIM47 antibody (STJ119831) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TRIM47 antibody (356-530) (STJ119831)

SKU:
STJ119831

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM47 (356-530) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM47
Gene ID: 91107
Uniprot ID: TRI47_HUMAN
Immunogen Region: 356-530
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 356-530 of human TRIM47 (NP_258411.2).
Immunogen Sequence: ELSFTKSSQAVRAVRDMLAV ACVNQWEQLRGPGGNEDGPQ KLDSEADAEPQDLESTNLLE SEAPRDYFLKFAYIVDLDSD TADKFLQLFGTKGVKRVLCP INYPLSPTRFTHCEQVLGEG ALDRGTYYWEVEIIEGWVSM GVMAEDFSPQEPYDRGRLGR NAHSCCLQWNGRSFS
Tissue Specificity Low expression in most tissues. Higher expression in kidney tubular cells. Overexpressed in astrocytoma tumor cells.
Function E3 ubiquitin-protein ligase that mediates the ubiquitination and proteasomal degradation of CYLD.
Protein Name E3 Ubiquitin-Protein Ligase Trim47
Gene Overexpressed In Astrocytoma Protein
Ring Finger Protein 100
Tripartite Motif-Containing Protein 47
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim47 antibody
Anti-Gene Overexpressed In Astrocytoma Protein antibody
Anti-Ring Finger Protein 100 antibody
Anti-Tripartite Motif-Containing Protein 47 antibody
Anti-TRIM47 antibody
Anti-GOA antibody
Anti-RNF100 antibody

Information sourced from Uniprot.org

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