• Immunofluorescence analysis of U2OS cells using TRIM4 Rabbit polyclonal antibody (STJ118367) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using TRIM4 antibody (STJ118367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TRIM4 antibody (1-280) (STJ118367)

SKU:
STJ118367

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM4
Gene ID: 89122
Uniprot ID: TRIM4_HUMAN
Immunogen Region: 1-280
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TRIM4 (NP_148977.2).
Immunogen Sequence: MEAEDIQEELTCPICLDYFQ DPVSIECGHNFCRGCLHRNW APGGGPFPCPECRHPSAPAA LRPNWALARLTEKTQRRRLG PVPPGLCGRHWEPLRLFCED DQRPVCLVCRESQEHQTHAM APIDEAFESYRTGNFDIHVD EWKRRLIRLLLYHFKQEEKL LKSQRNLVAKMKKVMHLQDV EVKNATQWKDKIKSQRMRIS TEFSKLHNFLVEEEDLFLQR LNKEEEETKKKLNENTLKL
Function E3 ubiquitin-protein ligase. Mediates 'Lys-63'-linked polyubiquitination of the innate immune receptor RIGI, this linkage doesn't lead to proteasomal degradation but seems to enhance IFN induction.
Protein Name E3 Ubiquitin-Protein Ligase Trim4
Ring Finger Protein 87
Ring-Type E3 Ubiquitin Transferase Trim4
Tripartite Motif-Containing Protein 4
Database Links Reactome: R-HSA-168928
Reactome: R-HSA-5689896
Reactome: R-HSA-918233
Reactome: R-HSA-933541
Reactome: R-HSA-933542
Reactome: R-HSA-933543
Reactome: R-HSA-936440
Reactome: R-HSA-9705671
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim4 antibody
Anti-Ring Finger Protein 87 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim4 antibody
Anti-Tripartite Motif-Containing Protein 4 antibody
Anti-TRIM4 antibody
Anti-RNF87 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance