• Western blot analysis of extracts of HeLa cells, using TRIM32 antibody (STJ29159) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using TRIM32 antibody (STJ29159) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TRIM32 antibody (1-280) (STJ29159)

SKU:
STJ29159

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM32 (1-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM32
Gene ID: 22954
Uniprot ID: TRI32_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TRIM32 (NP_036342.2).
Immunogen Sequence: MAAAAASHLNLDALREVLEC PICMESFTEEQLRPKLLHCG HTICRQCLEKLLASSINGVR CPFCSKITRITSLTQLTDNL TVLKIIDTAGLSEAVGLLMC RSCGRRLPRQFCRSCGLVLC EPCREADHQPPGHCTLPVKE AAEERRRDFGEKLTRLRELM GELQRRKAALEGVSKDLQAR YKAVLQEYGHEERRVQDELA RSRKFFTGSLAEVEKSNSQV VEEQSYLLNIAEVQAVSRC
Tissue Specificity Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.
Post Translational Modifications Ubiquitinated.
Function Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha. Also acts as a regulator of autophagy by mediating formation of unanchored 'Lys-63'-linked polyubiquitin chains that activate ULK1: interaction with AMBRA1 is required for ULK1 activation. (Microbial infection) May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
Protein Name E3 Ubiquitin-Protein Ligase Trim32
72 Kda Tat-Interacting Protein
Ring-Type E3 Ubiquitin Transferase Trim32
Tripartite Motif-Containing Protein 32
Zinc Finger Protein Ht2a
Database Links Reactome: R-HSA-3134975
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Localized In Cytoplasmic Bodies
Often Located Around The Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim32 antibody
Anti-72 Kda Tat-Interacting Protein antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim32 antibody
Anti-Tripartite Motif-Containing Protein 32 antibody
Anti-Zinc Finger Protein Ht2a antibody
Anti-TRIM32 antibody
Anti-HT2A antibody

Information sourced from Uniprot.org

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