• Western blot analysis of lysates from U-87MG cells, using TRHR Rabbit polyclonal antibody (STJ117429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-TRHR antibody (320-398) (STJ117429)

SKU:
STJ117429

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRHR (320-398) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRHR
Gene ID: 7201
Uniprot ID: TRFR_HUMAN
Immunogen Region: 320-398
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 320-398 of human TRHR (NP_003292.1).
Immunogen Sequence: YNLMSQKFRAAFRKLCNCKQ KPTEKPANYSVALNYSVIKE SDHFSTELDDITVTDTYLSA TKVSFDDTCLASEVSFSQS
Function Receptor for thyrotropin-releasing hormone (TRH). Upon ligand binding, this G-protein-coupled receptor triggers activation of the phosphatidylinositol (IP3)-calcium-protein kinase C (PKC) pathway.
Protein Name Thyrotropin-Releasing Hormone Receptor
Trh-R
Thyroliberin Receptor
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Thyrotropin-Releasing Hormone Receptor antibody
Anti-Trh-R antibody
Anti-Thyroliberin Receptor antibody
Anti-TRHR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance