Confocal imaging of U-2 OS cells using Transportin 3 (TNPO3) Rabbit monoclonal antibody (STJ11103126, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-Transportin 3 antibody (824-923) [S6MR] (STJ11103126)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103126
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General Information

Short DescriptionRabbit monoclonal Transportin 3 (TNPO3) (824-923) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS6MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTNPO3
Gene ID23534
Uniprot IDTNPO3_HUMAN
Immunogen Region824-923
Immunogen SequenceQVMNQLGQQLVSQLLHTCCF CLPPYTLPDVAEVLWEIMQV DRPTFCRWLENSLKGLPKET TVGAVTVTHKQLTDFHKQVT SAEECKQVCWALRDFTRLFR
SpecificityA synthetic peptide corresponding to a sequence within amino acids 824-923 of human Transportin 3 (Transportin 3 (TNPO3) ) (Q9Y5L0).

Additional Info

Tissue Specificity Expressed in skeletal muscle.
Function Importin, which transports target proteins into the nucleus. Specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1 and SFRS2, by recognizing phosphorylated SR domains. Also mediates the nuclear import of serine/arginine (SR) protein CPSF6, independently of CPSF6 phosphorylation. The nuclear import process is regulated by the small GTPase Ran that partitions between cytoplasm and nucleus in the predominantly GDP- and GTP-bound form, respectively. Importin associates with target cargo proteins in the cytoplasm, and the competitive binding of GTP-bound Ran induces the release of cargos in the nucleus. (Microbial infection) Involved in immunodeficiency virus (HIV-1) infection by importing the pre-integration complex (PIC) into the nucleus. Required for a nuclear maturation step of HIV-1 prior to integration.
Protein Name Transportin-3
Importin-12
Imp12
Transportin-Sr
Trn-Sr
Database Links
Cellular Localisation Nucleus Envelope
Cytoplasm
Localizes To The Nuclear Envelope And Annulate Lamellae
Which Consists In Stacks Of Endoplasmic Reticulum Membranes Containing A High Density Of Nuclear Pores
Alternative Antibody Names Anti-Transportin-3 antibody
Anti-Importin-12 antibody
Anti-Imp12 antibody
Anti-Transportin-Sr antibody
Anti-Trn-Sr antibody
Anti-TNPO3 antibody
Anti-IPO12 antibody

Information sourced from Uniprot.org

Citations

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