• Confocal imaging of U-2 OS cells using Transportin 3 (TNPO3) Rabbit monoclonal antibody (STJ11103126, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of NIH/3T3 cells using Transportin 3 (TNPO3) Rabbit monoclonal antibody (STJ11103126, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of paraffin-embedded Mouse kidney tissue using Transportin 3 (TNPO3) Rabbit monoclonal antibody (STJ11103126, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Transportin 3 (TNPO3) in paraffin-embedded human esophageal cancer using Transportin 3 (Transportin 3 (TNPO3) ) Rabbit monoclonal antibody (STJ11103126) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Transportin 3 (TNPO3) in paraffin-embedded rat kidney using Transportin 3 (Transportin 3 (TNPO3) ) Rabbit monoclonal antibody (STJ11103126) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Transportin 3 (Transportin 3 (TNPO3) ) Rabbit monoclonal antibody (STJ11103126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Transportin 3 antibody (824-923) [S6MR] (STJ11103126)

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STJ11103126

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Transportin 3 (TNPO3) (824-923) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNPO3
Gene ID: 23534
Uniprot ID: TNPO3_HUMAN
Immunogen Region: 824-923
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 824-923 of human Transportin 3 (Transportin 3 (TNPO3) ) (Q9Y5L0).
Immunogen Sequence: QVMNQLGQQLVSQLLHTCCF CLPPYTLPDVAEVLWEIMQV DRPTFCRWLENSLKGLPKET TVGAVTVTHKQLTDFHKQVT SAEECKQVCWALRDFTRLFR
Tissue Specificity Expressed in skeletal muscle.
Function Importin, which transports target proteins into the nucleus. Specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1 and SFRS2, by recognizing phosphorylated SR domains. Also mediates the nuclear import of serine/arginine (SR) protein CPSF6, independently of CPSF6 phosphorylation. The nuclear import process is regulated by the small GTPase Ran that partitions between cytoplasm and nucleus in the predominantly GDP- and GTP-bound form, respectively. Importin associates with target cargo proteins in the cytoplasm, and the competitive binding of GTP-bound Ran induces the release of cargos in the nucleus. (Microbial infection) Involved in immunodeficiency virus (HIV-1) infection by importing the pre-integration complex (PIC) into the nucleus. Required for a nuclear maturation step of HIV-1 prior to integration.
Protein Name Transportin-3
Importin-12
Imp12
Transportin-Sr
Trn-Sr
Cellular Localisation Nucleus Envelope
Cytoplasm
Localizes To The Nuclear Envelope And Annulate Lamellae
Which Consists In Stacks Of Endoplasmic Reticulum Membranes Containing A High Density Of Nuclear Pores
Alternative Antibody Names Anti-Transportin-3 antibody
Anti-Importin-12 antibody
Anti-Imp12 antibody
Anti-Transportin-Sr antibody
Anti-Trn-Sr antibody
Anti-TNPO3 antibody
Anti-IPO12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance