Anti-Transcription Initiation Factor IIB antibody [R06-4C2] (STJA0035381)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human TFIIB
General Information
| Short Description | Rabbit monoclonal anti-Transcription Initiation Factor IIB for use in WB and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R06-4C2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | GTF2B |
| Gene ID | 2959 |
| Uniprot ID | TF2B_HUMAN |
| Immunogen | A synthetic peptide of human TFIIB |
Additional Info
| Tissue Specificity | Expressed in the inner cell mass forming the embryoblast. Not detected in cells from the outer thin layer trophoblast (at protein level). |
| Post Translational Modifications | Acetylated. Autoacetylated.autoacetylation at Lys-238 stimulates transcription activation. |
| Function | General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner.these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-GCGCGACGCC-3', and downstream (BREd), 5'-GATTGATGGTTGTG-3', of the TATA box element. Modulates transcription start site selection. Also exhibits autoacetyltransferase activity that contributes to the activated transcription. |
| Protein Name | Transcription Initiation Factor IibGeneral Transcription Factor TfiibS300-Ii |
| Database Links | Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-674695Reactome: R-HSA-6807505Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-76042 |
| Cellular Localisation | NucleusChromosomeNon-Acetylated Form Colocalizes With Dna In The G0/1S And G2 Phases Of The Cell CycleBut Not During MitosisAcetylated Form Colocalizes At Transcriptionally Silent Mitotic Chromatids During Mitosis At MetaphaseAnaphaseAnd Telophase Phases Of The Cell Cycle |
| Alternative Antibody Names | Anti-Transcription Initiation Factor Iib antibodyAnti-General Transcription Factor Tfiib antibodyAnti-S300-Ii antibodyAnti-GTF2B antibodyAnti-TF2B antibodyAnti-TFIIB antibody |
Information sourced from Uniprot.org