Western blot analysis of extracts of various cell lines, using TPP1 antibody (STJ27594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TPP1 antibody (284-563) (STJ27594)

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STJ27594
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPP1
Gene ID: 1200
Uniprot ID: TPP1_HUMAN
Immunogen Region: 284-563
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 284-563 of human TPP1 (NP_000382.3).
Immunogen Sequence: GANISTWVYSSPGRHEGQEP FLQWLMLLSNESALPHVHTV SYGDDEDSLSSAYIQRVNTE LMKAAARGLTLLFASGDSGA GCWSVSGRHQFRPTFPASSP YVTTVGGTSFQEPFLITNEI VDYISGGGFSNVFPRPSYQE EAVTKFLSSSPHLPPSSYFN ASGRAYPDVAALSDGYWVVS NRVPIPWVSGTSASTPVFGG ILSLINEHRILSGRPPLGFL NPRLYQQHGAGLFDVTRGC
Tissue Specificity Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.
Post Translational Modifications Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity.
Function Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.
Protein Name Tripeptidyl-Peptidase 1
Tpp-1
Cell Growth-Inhibiting Gene 1 Protein
Lysosomal Pepstatin-Insensitive Protease
Lpic
Tripeptidyl Aminopeptidase
Tripeptidyl-Peptidase I
Tpp-I
Database Links Reactome: R-HSA-381038
Cellular Localisation Lysosome
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Tripeptidyl-Peptidase 1 antibody
Anti-Tpp-1 antibody
Anti-Cell Growth-Inhibiting Gene 1 Protein antibody
Anti-Lysosomal Pepstatin-Insensitive Protease antibody
Anti-Lpic antibody
Anti-Tripeptidyl Aminopeptidase antibody
Anti-Tripeptidyl-Peptidase I antibody
Anti-Tpp-I antibody
Anti-TPP1 antibody
Anti-CLN2 antibody
Anti-GIG1 antibody
Anti-UNQ267 antibody
Anti-PRO304 antibody

Information sourced from Uniprot.org

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