• Western blot analysis of extracts of various cell lines, using MCCC2 antibody (STJ110297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of MCCC2 in paraffin-embedded mouse kidney tissue using MCCC2 Rabbit polyclonal antibody (STJ110297) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MCCC2 in paraffin-embedded rat kidney tissue using MCCC2 Rabbit polyclonal antibody (STJ110297) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MCCC2 in paraffin-embedded human colon tissue using MCCC2 Rabbit polyclonal antibody (STJ110297) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MCCC2 in paraffin-embedded human liver tissue using MCCC2 Rabbit polyclonal antibody (STJ110297) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using MCCC2 Rabbit polyclonal antibody (STJ110297) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : MOLT-4. Exposure time: 30s.

Anti-MCCC2 antibody (284-563) (STJ110297)

SKU:
STJ110297

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MCCC2 (284-563) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCCC2
Gene ID: 64087
Uniprot ID: MCCB_HUMAN
Immunogen Region: 284-563
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 284-563 of human MCCC2 (NP_071415.1).
Immunogen Sequence: LHLTRKVVRNLNYQKKLDVT IEPSEEPLFPADELYGIVGA NLKRSFDVREVIARIVDGSR FTEFKAFYGDTLVTGFARIF GYPVGIVGNNGVLFSESAKK GTHFVQLCCQRNIPLLFLQN ITGFMVGREYEAEGIAKDGA KMVAAVACAQVPKITLIIGG SYGAGNYGMCGRAYSPRFLY IWPNARISVMGGEQAANVLA TITKDQRAREGKQFSSADEA ALKEPIIKKFEEEGNPYYS
Function Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.
Protein Name Methylcrotonoyl-Coa Carboxylase Beta Chain - Mitochondrial
Mccase Subunit Beta
3-Methylcrotonyl-Coa Carboxylase 2
3-Methylcrotonyl-Coa Carboxylase Non-Biotin-Containing Subunit
3-Methylcrotonyl-Coa -Carbon Dioxide Ligase Subunit Beta
Database Links Reactome: R-HSA-196780
Reactome: R-HSA-3371599
Reactome: R-HSA-70895
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Methylcrotonoyl-Coa Carboxylase Beta Chain - Mitochondrial antibody
Anti-Mccase Subunit Beta antibody
Anti-3-Methylcrotonyl-Coa Carboxylase 2 antibody
Anti-3-Methylcrotonyl-Coa Carboxylase Non-Biotin-Containing Subunit antibody
Anti-3-Methylcrotonyl-Coa -Carbon Dioxide Ligase Subunit Beta antibody
Anti-MCCC2 antibody
Anti-MCCB antibody

Information sourced from Uniprot.org

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