Anti-TPMT antibody (1-245) (STJ25944)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TPMT (1-245) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:10-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TPMT |
| Gene ID | 7172 |
| Uniprot ID | TPMT_HUMAN |
| Immunogen Region | 1-245 |
| Immunogen Sequence | MDGTRTSLDIEEYSDTEVQK NQVLTLEEWQDKWVNGKTAF HQEQGHQLLKKHLDTFLKGK SGLRVFFPLCGKAVEMKWFA DRGHSVVGVEISELGIQEFF TEQNLSYSEEPITEIPGTKV FKSSSGNISLYCCSIFDLPR TNIGKFDMIWDRGALVAINP GDRKCYADTMFSLLGKKFQY LLCVLSYDPTKHPGPPFYVP HAEIERLFGKICNIRCLEKV DAFEERHKSWGIDCLFEKL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPMT (NP_000358.1). |
Additional Info
| Function | Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor. TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified. |
| Protein Name | Thiopurine S-MethyltransferaseThiopurine Methyltransferase |
| Database Links | Reactome: R-HSA-156581Reactome: R-HSA-5578995Reactome: R-HSA-9748787 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Thiopurine S-Methyltransferase antibodyAnti-Thiopurine Methyltransferase antibodyAnti-TPMT antibody |
Information sourced from Uniprot.org