Anti-TPM1 antibody (1-245) (STJ25940)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TPM1 (1-245) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TPM1 |
| Gene ID | 7168 |
| Uniprot ID | TPM1_HUMAN |
| Immunogen Region | 1-245 |
| Immunogen Sequence | MAGSSSLEAVRRKIRSLQEQ ADAAEERAGTLQRELDHERK LRETAEADVASLNRRIQLVE EELDRAQERLATALQKLEEA EKAADESERGMKVIESRAQK DEEKMEIQEIQLKEAKHIAE DADRKYEEVARKLVIIESDL ERAEERAELSEGKCAELEEE LKTVTNNLKSLEAQAEKYSQ KEDRYEEEIKVLSDKLKEAE TRAEFAERSVTKLEKSIDDL EDQLYQQLEQNRRLTNELK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human Tropomyosin 1 (NP_001018008.1). |
Additional Info
| Tissue Specificity | Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle. |
| Post Translational Modifications | Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells. |
| Function | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. |
| Protein Name | Tropomyosin Alpha-1 ChainAlpha-TropomyosinTropomyosin-1 |
| Database Links | Reactome: R-HSA-390522Reactome: R-HSA-445355 |
| Cellular Localisation | CytoplasmCytoskeletonAssociates With F-Actin Stress Fibers |
| Alternative Antibody Names | Anti-Tropomyosin Alpha-1 Chain antibodyAnti-Alpha-Tropomyosin antibodyAnti-Tropomyosin-1 antibodyAnti-TPM1 antibodyAnti-C15orf13 antibodyAnti-TMSA antibody |
Information sourced from Uniprot.org