| Host: |
Rabbit |
| Applications: |
WB/IF/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TPM1 (1-245) is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TPM1 |
| Gene ID: |
7168 |
| Uniprot ID: |
TPM1_HUMAN |
| Immunogen Region: |
1-245 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human Tropomyosin 1 (NP_001018008.1). |
| Immunogen Sequence: |
MAGSSSLEAVRRKIRSLQEQ ADAAEERAGTLQRELDHERK LRETAEADVASLNRRIQLVE EELDRAQERLATALQKLEEA EKAADESERGMKVIESRAQK DEEKMEIQEIQLKEAKHIAE DADRKYEEVARKLVIIESDL ERAEERAELSEGKCAELEEE LKTVTNNLKSLEAQAEKYSQ KEDRYEEEIKVLSDKLKEAE TRAEFAERSVTKLEKSIDDL EDQLYQQLEQNRRLTNELK |
| Tissue Specificity | Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle. |
| Post Translational Modifications | Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells. |
| Function | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. |
| Protein Name | Tropomyosin Alpha-1 ChainAlpha-TropomyosinTropomyosin-1 |
| Database Links | Reactome: R-HSA-390522Reactome: R-HSA-445355 |
| Cellular Localisation | CytoplasmCytoskeletonAssociates With F-Actin Stress Fibers |
| Alternative Antibody Names | Anti-Tropomyosin Alpha-1 Chain antibodyAnti-Alpha-Tropomyosin antibodyAnti-Tropomyosin-1 antibodyAnti-TPM1 antibodyAnti-C15orf13 antibodyAnti-TMSA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
