• Western blot analysis of extracts of various cell lines, using TPK1 antibody (STJ25939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TPK1 antibody (1-243) (STJ25939)

SKU:
STJ25939

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TPK1 (1-243) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPK1
Gene ID: 27010
Uniprot ID: TPK1_HUMAN
Immunogen Region: 1-243
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-243 of human TPK1 (NP_071890.2).
Immunogen Sequence: MEHAFTPLEPLLSTGNLKYC LVILNQPLDNYFRHLWNKAL LRACADGGANRLYDITEGER ESFLPEFINGDFDSIRPEVR EYYATKGCELISTPDQDHTD FTKCLKMLQKKIEEKDLKVD VIVTLGGLAGRFDQIMASVN TLFQATHITPFPIIIIQEES LIYLLQPGKHRLHVDTGMEG DWCGLIPVGQPCMQVTTTGL KWNLTNDVLAFGTLVSTSNT YDGSGVVTVETDHPLLWTM
Tissue Specificity Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.
Function Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
Protein Name Thiamin Pyrophosphokinase 1
Htpk1
Placental Protein 20
Pp20
Thiamine Pyrophosphokinase 1
Database Links Reactome: R-HSA-196819
Alternative Antibody Names Anti-Thiamin Pyrophosphokinase 1 antibody
Anti-Htpk1 antibody
Anti-Placental Protein 20 antibody
Anti-Pp20 antibody
Anti-Thiamine Pyrophosphokinase 1 antibody
Anti-TPK1 antibody

Information sourced from Uniprot.org

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