• Immunohistochemistry analysis of paraffin-embedded rat brain using TPD52 antibody (STJ112292) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using TPD52 antibody (STJ112292) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TPD52 antibody (STJ112292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TPD52 antibody (1-184) (STJ112292)

SKU:
STJ112292

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TPD52 (1-184) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPD52
Gene ID: 7163
Uniprot ID: TPD52_HUMAN
Immunogen Region: 1-184
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human TPD52 (NP_005070.1).
Immunogen Sequence: MDRGEQGLLRTDPVPEEGED VAATISATETLSEEEQEELR RELAKVEEEIQTLSQVLAAK EKHLAEIKRKLGINSLQELK QNIAKGWQDVTATSAYKKTS ETLSQAGQKASAAFSSVGSV ITKKLEDVKNSPTFKSFEEK VENLKSKVGGTKPAGGDFGE VLNSAANASATTTEPLPEKT QESL
Tissue Specificity Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
Protein Name Tumor Protein D52
Protein N8
Database Links Reactome: R-HSA-432722
Alternative Antibody Names Anti-Tumor Protein D52 antibody
Anti-Protein N8 antibody
Anti-TPD52 antibody

Information sourced from Uniprot.org

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