• Western blot analysis of lysates from HepG2, COLO205, and HUVEC cells, using TOP3B Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Topo III Beta-1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-TOP3B antibody (51-100 aa) (STJ96063)

SKU:
STJ96063

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA topoisomerase 3-beta-1 (51-100 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOP3B
Gene ID: 8940
Uniprot ID: TOP3B_HUMAN
Immunogen Region: 51-100 aa
Specificity: Topo III Beta-1 Polyclonal Antibody detects endogenous levels of Topo III Beta-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human TOP3B at the amino acid range 51-100
Function Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Possesses negatively supercoiled DNA relaxing activity.
Protein Name Dna Topoisomerase 3-Beta-1
Dna Topoisomerase Iii Beta-1
Alternative Antibody Names Anti-Dna Topoisomerase 3-Beta-1 antibody
Anti-Dna Topoisomerase Iii Beta-1 antibody
Anti-TOP3B antibody
Anti-TOP3B1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance