| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-DNA topoisomerase 3-beta-1 (51-100 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TOP3B |
| Gene ID: | 8940 |
| Uniprot ID: | TOP3B_HUMAN |
| Immunogen Region: | 51-100 aa |
| Specificity: | Topo III Beta-1 Polyclonal Antibody detects endogenous levels of Topo III Beta-1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human TOP3B at the amino acid range 51-100 |
| Function | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Possesses negatively supercoiled DNA relaxing activity. |
| Protein Name | Dna Topoisomerase 3-Beta-1Dna Topoisomerase Iii Beta-1 |
| Alternative Antibody Names | Anti-Dna Topoisomerase 3-Beta-1 antibodyAnti-Dna Topoisomerase Iii Beta-1 antibodyAnti-TOP3B antibodyAnti-TOP3B1 antibody |
Information sourced from Uniprot.org