• Immunohistochemistry analysis of TOP1MT in paraffin-embedded Rat lung using TOP1MT Rabbit polyclonal antibody (STJ117754) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TOP1MT in paraffin-embedded Human colon using TOP1MT Rabbit polyclonal antibody (STJ117754) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from A-549 cells, using TOP1MT Rabbit polyclonal antibody (STJ117754) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60S.

Anti-TOP1MT antibody (470-550) (STJ117754)

SKU:
STJ117754

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TOP1MT (470-550) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOP1MT
Gene ID: 116447
Uniprot ID: TOP1M_HUMAN
Immunogen Region: 470-550
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1).
Immunogen Sequence: RATPSTFEKSMQNLQTKIQA KKEQVAEARAELRRARAEHK AQGDGKSRSVLEKKRRLLEK LQEQLAQLSVQATDKEENKQ V
Tissue Specificity Ubiquitous.highest in skeletal muscle, heart, brain and fetal liver.
Function Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
Protein Name Dna Topoisomerase I - Mitochondrial
Top1mt
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Dna Topoisomerase I - Mitochondrial antibody
Anti-Top1mt antibody
Anti-TOP1MT antibody

Information sourced from Uniprot.org

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