Anti-TOP1MT antibody (470-550) (STJ117754)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TOP1MT (470-550) antibody for use in WB, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TOP1MT |
| Gene ID | 116447 |
| Uniprot ID | TOP1M_HUMAN |
| Immunogen Region | 470-550 |
| Immunogen Sequence | RATPSTFEKSMQNLQTKIQA KKEQVAEARAELRRARAEHK AQGDGKSRSVLEKKRRLLEK LQEQLAQLSVQATDKEENKQ V |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1). |
Additional Info
| Tissue Specificity | Ubiquitous.highest in skeletal muscle, heart, brain and fetal liver. |
| Function | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. |
| Protein Name | Dna Topoisomerase I - MitochondrialTop1mt |
| Database Links | |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Dna Topoisomerase I - Mitochondrial antibodyAnti-Top1mt antibodyAnti-TOP1MT antibody |
Information sourced from Uniprot.org