• Confocal immunofluorescence analysis of U2OS cells using TOMM40 Polyclonal Antibody (STJ25917) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using TOMM40 antibody (STJ25917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TOMM40 antibody (1-90) (STJ25917)

SKU:
STJ25917

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TOMM40 (1-90) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOMM40
Gene ID: 10452
Uniprot ID: TOM40_HUMAN
Immunogen Region: 1-90
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human TOMM40 (NP_001122389.1).
Immunogen Sequence: MGNVLAASSPPAGPPPPPAP ALVGLPPPPPSPPGFTLPPL GGSLGAGTSTSRSSERTPGA ATASASGAAEDGACGCLPNP GTFEECHRKC
Function Channel-forming protein essential for import of protein precursors into mitochondria. Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria.
Protein Name Mitochondrial Import Receptor Subunit Tom40 Homolog
Protein Haymaker
Translocase Of Outer Membrane 40 Kda Subunit Homolog
P38.5
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-5205685
Cellular Localisation Mitochondrion Outer Membrane
Multi-Pass Membrane Protein
Associates With The Mitochondria-Associated Er Membrane Via Interaction With Bcap31
Alternative Antibody Names Anti-Mitochondrial Import Receptor Subunit Tom40 Homolog antibody
Anti-Protein Haymaker antibody
Anti-Translocase Of Outer Membrane 40 Kda Subunit Homolog antibody
Anti-P38.5 antibody
Anti-TOMM40 antibody
Anti-C19orf1 antibody
Anti-PEREC1 antibody
Anti-TOM40 antibody

Information sourced from Uniprot.org

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