• Immunofluorescence analysis of L929 cells using TOLLIP antibody (STJ25913) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using TOLLIP antibody (STJ25913) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TOLLIP Rabbit polyclonal antibody antibody (STJ25913) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TOLLIP antibody (1-274) (STJ25913)

SKU:
STJ25913

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TOLLIP (1-274) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOLLIP
Gene ID: 54472
Uniprot ID: TOLIP_HUMAN
Immunogen Region: 1-274
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human TOLLIP (NP_061882.2).
Immunogen Sequence: MATTVSTQRGPVYIGELPQD FLRITPTQQQRQVQLDAQAA QQLQYGGAVGTVGRLNITVV QAKLAKNYGMTRMDPYCRLR LGYAVYETPTAHNGAKNPRW NKVIHCTVPPGVDSFYLEIF DERAFSMDDRIAWTHITIPE SLRQGKVEDKWYSLSGRQGD DKEGMINLVMSYALLPAAMV MPPQPVVLMPTVYQQGVGYV PITGMPAVCSPGMVPVALPP AAVNAQPRCSEEDLKAIQD
Post Translational Modifications Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.
Function Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates. In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P).
Protein Name Toll-Interacting Protein
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-9020702
Cellular Localisation Cytoplasm
Endosome
Early Endosome
Localized To Endo/Exosomal Vesicles
Alternative Antibody Names Anti-Toll-Interacting Protein antibody
Anti-TOLLIP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance