• Western blot analysis of extracts of various cell lines, using TNPO1 antibody (STJ111699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using TNPO1 antibody (STJ111699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.

Anti-TNPO1 antibody (790-890) (STJ111699)

SKU:
STJ111699

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNPO1 (790-890) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNPO1
Gene ID: 3842
Uniprot ID: TNPO1_HUMAN
Immunogen Region: 790-890
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 790-890 of human TNPO1 (NP_002261.3).
Immunogen Sequence: CPQEVAPMLQQFIRPWCTSL RNIRDNEEKDSAFRGICTMI SVNPSGVIQDFIFFCDAVAS WINPKDDLRDMFCKILHGFK NQVGDENWRRFSDQFPLPLK E
Function Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. May mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates nuclear import of SRP19. Mediates nuclear import of ADAR/ADAR1 isoform 1 and isoform 5 in a RanGTP-dependent manner. (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
Protein Name Transportin-1
Importin Beta-2
Karyopherin Beta-2
M9 Region Interaction Protein
Mip
Database Links Reactome: R-HSA-450513
Reactome: R-HSA-5620924
Reactome: R-HSA-9615933
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Transportin-1 antibody
Anti-Importin Beta-2 antibody
Anti-Karyopherin Beta-2 antibody
Anti-M9 Region Interaction Protein antibody
Anti-Mip antibody
Anti-TNPO1 antibody
Anti-KPNB2 antibody
Anti-MIP1 antibody
Anti-TRN antibody

Information sourced from Uniprot.org

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