• Immunohistochemistry analysis of paraffin-embedded mouse lung using TNNC2 antibody (STJ110051) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using TNNC2 antibody (STJ110051) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TNNC2 antibody (STJ110051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Anti-TNNC2 antibody (1-160) (STJ110051)

SKU:
STJ110051

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNNC2 (1-160) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNNC2
Gene ID: 7125
Uniprot ID: TNNC2_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TNNC2 (NP_003270.1).
Immunogen Sequence: MTDQQAEARSYLSEEMIAEF KAAFDMFDADGGGDISVKEL GTVMRMLGQTPTKEELDAII EEVDEDGSGTIDFEEFLVMM VRQMKEDAKGKSEEELAECF RIFDRNADGYIDPEELAEIF RASGEHVTDEEIESLMKDGD KNNDGRIDFDEFLKMMEGVQ
Function Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
Protein Name Troponin C - Skeletal Muscle
Database Links Reactome: R-HSA-390522
Alternative Antibody Names Anti-Troponin C - Skeletal Muscle antibody
Anti-TNNC2 antibody

Information sourced from Uniprot.org

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