• Immunofluorescence analysis of L929 cells using TNFRSF11A Rabbit polyclonal antibody (STJ114966) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using TNFRSF11A Rabbit polyclonal antibody (STJ114966) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TNFRSF11A antibody (466-616) (STJ114966)

SKU:
STJ114966

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNFRSF11A (466-616) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:100-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFRSF11A
Gene ID: 8792
Uniprot ID: TNR11_HUMAN
Immunogen Region: 466-616
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 466-616 of human TNFRSF11A (NP_003830.1).
Immunogen Sequence: RGPLPQCAYGMGLPPEEEAS RTEARDQPEDGADGRLPSSA RAGAGSGSSPGGQSPASGNV TGNSNSTFISSGQVMNFKGD IIVVYVSQTSQEGAAAAAEP MGRPVQEETLARRDSFAGNG PRFPDPCGGPEGLREPEKAS RPVQEQGGAKA
Tissue Specificity Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
Function Receptor for TNFSF11/RANKL/TRANCE/OPGL.essential for RANKL-mediated osteoclastogenesis. Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2. Involved in the regulation of interactions between T-cells and dendritic cells.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 11a
Osteoclast Differentiation Factor Receptor
Odfr
Receptor Activator Of Nf-Kb
Cd Antigen Cd265
Database Links Reactome: R-HSA-5668541
Reactome: R-HSA-5676594
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Isoform Rank-E5a: Cell Membrane
Alternative Antibody Names Anti-Tumor Necrosis Factor Receptor Superfamily Member 11a antibody
Anti-Osteoclast Differentiation Factor Receptor antibody
Anti-Odfr antibody
Anti-Receptor Activator Of Nf-Kb antibody
Anti-Cd Antigen Cd265 antibody
Anti-TNFRSF11A antibody
Anti-RANK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance