• Confocal imaging of NIH-3T3 cells using DR5/TRAILR2/TNFRSF10B Rabbit monoclonal antibody (STJ11101581, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using DR5/TRAILR2/TNFRSF10B Rabbit monoclonal antibody (STJ11101581) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DR5/TRAILR2/TNFRSF10B antibody (STJ11101581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of Mouse heart, using DR5/TRAILR2/TNFRSF10B antibody (STJ11101581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TNFRSF10B antibody (341-440) [S1MR] (STJ11101581)

SKU:
STJ11101581

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DR5 (341-440) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFRSF10B
Gene ID: 8795
Uniprot ID: TR10B_HUMAN
Immunogen Region: 341-440
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 341-440 of human DR5/TRAILR2/TNFRSF10B (O14763).
Immunogen Sequence: CFDDFADLVPFDSWEPLMRK LGLMDNEIKVAKAEAAGHRD TLYTMLIKWVNKTGRDASVH TLLDALETLGERLAKQKIED HLLSSGKFMYLEGNADSAMS
Tissue Specificity Widely expressed in adult and fetal tissues.very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361.highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract.not detectable in brain.
Post Translational Modifications (Microbial infection) Glycosylated on Arg residue by S.typhimurium protein Ssek3.
Function Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 10b
Death Receptor 5
Tnf-Related Apoptosis-Inducing Ligand Receptor 2
Trail Receptor 2
Trail-R2
Cd Antigen Cd262
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-202733
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-6803211
Reactome: R-HSA-69416
Reactome: R-HSA-75158
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tumor Necrosis Factor Receptor Superfamily Member 10b antibody
Anti-Death Receptor 5 antibody
Anti-Tnf-Related Apoptosis-Inducing Ligand Receptor 2 antibody
Anti-Trail Receptor 2 antibody
Anti-Trail-R2 antibody
Anti-Cd Antigen Cd262 antibody
Anti-TNFRSF10B antibody
Anti-DR5 antibody
Anti-KILLER antibody
Anti-TRAILR2 antibody
Anti-TRICK2 antibody
Anti-ZTNFR9 antibody
Anti-UNQ160 antibody
Anti-PRO186 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance