• Western blot analysis of extracts of DU145 cells, using TNFRSF10A antibody (STJ28189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TNFRSF10A antibody (269-468) (STJ28189)

SKU:
STJ28189

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNFRSF10A (269-468) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFRSF10A
Gene ID: 8797
Uniprot ID: TR10A_HUMAN
Immunogen Region: 269-468
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 269-468 of human TNFRSF10A (NP_003835.3).
Immunogen Sequence: GGDPKCMDRVCFWRLGLLRG PGAEDNAHNEILSNADSLST FVSEQQMESQEPADLTGVTV QSPGEAQCLLGPAEAEGSQR RRLLVPANGADPTETLMLFF DKFANIVPFDSWDQLMRQLD LTKNEIDVVRAGTAGPGDAL YAMLMKWVNKTGRNASIHTL LDALERMEERHAREKIQDLL VDSGKFIYLEDGTGSAVSLE
Tissue Specificity Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells.
Post Translational Modifications Palmitoylated. Palmitoylation of TNFRSF10A is required for its association with lipid rafts, oligomerization and function in TRAIL-induced cell death. Palmitoylated by ZDHHC3 (Probable).
Function Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 10a
Death Receptor 4
Tnf-Related Apoptosis-Inducing Ligand Receptor 1
Trail Receptor 1
Trail-R1
Cd Antigen Cd261
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-202733
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-6803211
Reactome: R-HSA-69416
Reactome: R-HSA-75158
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Cytoplasm
Cytosol
Palmitoylation Is Required For Association With Membranes
Alternative Antibody Names Anti-Tumor Necrosis Factor Receptor Superfamily Member 10a antibody
Anti-Death Receptor 4 antibody
Anti-Tnf-Related Apoptosis-Inducing Ligand Receptor 1 antibody
Anti-Trail Receptor 1 antibody
Anti-Trail-R1 antibody
Anti-Cd Antigen Cd261 antibody
Anti-TNFRSF10A antibody
Anti-APO2 antibody
Anti-DR4 antibody
Anti-TRAILR1 antibody

Information sourced from Uniprot.org

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