Anti-TNFAIP3 antibody (321-370 aa) (STJ91402)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TNAP3 at the amino acid range 321-370
General Information
| Short Description | Rabbit polyclonal anti-Tumor necrosis factor alpha-induced protein 3 (321-370 aa) for use in WB, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TNFAIP3 |
| Gene ID | 7128 |
| Uniprot ID | TNAP3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TNAP3 at the amino acid range 321-370 |
| Immunogen Region | 321-370 aa |
| Specificity | A20 Polyclonal Antibody detects endogenous levels of A20 protein. |
Additional Info
| Function | Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways.affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG.the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin.polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of pro-inflammatory cytokines and IFN beta in LPS-tolerized macrophages. |
| Protein Name | Tumor Necrosis Factor Alpha-Induced Protein 3Tnf Alpha-Induced Protein 3Otu Domain-Containing Protein 7cPutative Dna-Binding Protein A20Zinc Finger Protein A20 Cleaved Into - A20p50 - A20p37 |
| Database Links | Reactome: R-HSA-168638Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5689896Reactome: R-HSA-936440 |
| Cellular Localisation | CytoplasmNucleusLysosomeA20p50: Cytoplasm |
| Alternative Antibody Names | Anti-Tumor Necrosis Factor Alpha-Induced Protein 3 antibodyAnti-Tnf Alpha-Induced Protein 3 antibodyAnti-Otu Domain-Containing Protein 7c antibodyAnti-Putative Dna-Binding Protein A20 antibodyAnti-Zinc Finger Protein A20 Cleaved Into - A20p50 - A20p37 antibodyAnti-TNFAIP3 antibodyAnti-OTUD7C antibody |
Information sourced from Uniprot.org