• Immunofluorescence analysis of mouse liver cells using TMPRSS6 Rabbit polyclonal antibody (STJ11103816) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat liver, using TMPRSS6 antibody (STJ11103816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using TMPRSS6 antibody (STJ11103816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TMPRSS6 antibody (77-220) (STJ11103816)

SKU:
STJ11103816

£116.50 - £543.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transmembrane Protease Serine 6 (77-220) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TMPRSS6
Gene ID: 164656
Uniprot ID: TMPS6_HUMAN
Immunogen Region: 77-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 77-220 of human TMPRSS6 (NP_705837.1).
Immunogen Sequence: GYKAEVMVSQVYSGSLRVLN RHFSQDLTRRESSAFRSETA KAQKMLKELITSTRLGTYYN SSSVYSFGEGPLTCFFWFIL QIPEHRRLMLSPEVVQALLV EELLSTVNSSAAVPYRAEYE VDPEGLVILEASVKDIAALN STLG
Post Translational Modifications The single-chain zymogen undergoes autoproteolytic processing. This results in TMPRSS6 shedding from the cell surface and conversion into an activated two-chains form which is released extracellularly. The process involves a trans-activation mechanism that requires TMPRSS6 oligomerization.
Function Membrane-bound serine protease. Through the cleavage of cell surface HJV, a regulator of the expression of the iron absorption-regulating hormone hepicidin/HAMP, plays a role in iron homeostasis.
Protein Name Transmembrane Protease Serine 6
Matriptase-2
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
The Processed
Activated Two-Chains Form Is Released In The Extracellular Space
Alternative Antibody Names Anti-Transmembrane Protease Serine 6 antibody
Anti-Matriptase-2 antibody
Anti-TMPRSS6 antibody
Anti-UNQ354 antibody
Anti-PRO618 antibody

Information sourced from Uniprot.org

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