• Western blot analysis of extracts of HeLa cells, using TMPO antibody (STJ25881) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TMPO rabbit polyclonal antibody (STJ25881) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using TMPO rabbit polyclonal antibody (STJ25881) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using TMPO rabbit polyclonal antibody (STJ25881) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using TMPO rabbit polyclonal antibody (STJ25881) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using TMPO rabbit polyclonal antibody (STJ25881) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using TMPO rabbit polyclonal antibody (STJ25881) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-TMPO antibody (1-410) (STJ25881)

SKU:
STJ25881

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TMPO (1-410) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TMPO
TMPO
Gene ID: 7112
7112
Uniprot ID: LAP2A_HUMAN
LAP2B_HUMAN
Immunogen Region: 1-410
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).
Immunogen Sequence: MPEFLEDPSVLTKDKLKSEL VANNVTLPAGEQRKDVYVQL YLQHLTARNRPPLPAGTNSK GPPDFSSDEEREPTPVLGSG AAAAGRSRAAVGRKATKKTD KPRQEDKDDLDVTELTNEDL LDQLVKYGVNPGPIVGTTRK LYEKKLLKLREQGTESRSST PLPTISSSAENTRQNGSNDS DRYSDNEEDSKIELKLEKRE PLKGRAKTPVTLKQRRVEHN QSYSQAGITETEWTSGSSK
Tissue Specificity P42166 - Expressed in many tissues. Most abundant in adult thymus and fetal liver.
P42167 - Expressed in many tissues. Most abundant in adult thymus and fetal liver.
Alternative Antibody Names Anti-Lamina-Associated Polypeptide 2 - Isoform Alpha antibody
Anti-Thymopoietin Isoform Alpha antibody
Anti-Tp Alpha antibody
Anti-Thymopoietin-Related Peptide Isoform Alpha antibody
Anti-Tprp Isoform Alpha Cleaved Into - Thymopoietin antibody
Anti-Tp antibody
Anti-Splenin - Thymopentin antibody
Anti-Tp5 antibody
Anti-TMPO antibody
Anti-LAP2 antibody
Anti-Lamina-Associated Polypeptide 2 - Isoforms Beta/Gamma antibody
Anti-Thymopoietin - Isoforms Beta/Gamma antibody
Anti-Tp Beta/Gamma antibody
Anti-Thymopoietin-Related Peptide Isoforms Beta/Gamma antibody
Anti-Tprp Isoforms Beta/Gamma Cleaved Into - Thymopoietin antibody
Anti-Tp antibody
Anti-Splenin - Thymopentin antibody
Anti-Tp5 antibody
Anti-TMPO antibody
Anti-LAP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance