| Short Description : | Rabbit polyclonal TMEM147 (83-98) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TMEM147 |
| Gene ID: | 10430 |
| Uniprot ID: | TM147_HUMAN |
| Immunogen Region: | 83-98 |
| Immunogen Sequence: | MTLFHFGNCFALAYFPYFIT YKCSGLSEYNAFWKCVQAGV TYLFVQLCKMLFLATFFPTW EGGIYDFIGEFMKASVDVAD LIGLNLVMSRNAGKGEYKIM |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TMEM147 (NP_116024.1). |
| Function | Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions. Also acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane. Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity. Regulates LBR localization to the nucleus inner membrane. |
| Protein Name | Bos Complex Subunit Tmem147Protein Nifie 14Transmembrane Protein 147 |
| Database Links | |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinNucleus MembraneCell Membrane |
| Alternative Antibody Names | Anti-Bos Complex Subunit Tmem147 antibodyAnti-Protein Nifie 14 antibodyAnti-Transmembrane Protein 147 antibodyAnti-TMEM147 antibody |
Information sourced from Uniprot.org
