| Host: |
Sheep |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Sheep polyclonal antibody anti-TMC1 (1st Cytoplasmic Loop) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
TMC1 |
| Gene ID: |
117531 |
| Uniprot ID: |
TMC1_HUMAN |
| Immunogen Region: |
1st Cytoplasmic Loop |
| Specificity: |
Specific for TMC1. |
| Immunogen: |
A synthetic peptide from the 1t cytoplasmic loop of human TMC1 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in mouse. |
| Function | Pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system. The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, and aided by several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, and the tip-link PCDH15. MET channel is activated by tension in the tip-link extending from the side wall of one stereocilium to the tip of the adjacent shorter stereocilium, where the channel is located. TMC1 MET channel is highly permeable to calcium and likely transports monovalent cations. Also involved in vestibular hair cells transduction current. |
| Protein Name | Transmembrane Channel-Like Protein 1Transmembrane Cochlear-Expressed Protein 1 |
| Database Links | Reactome: R-HSA-9662360Reactome: R-HSA-9662361 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLocalized To The Stereocilia Tips Of The Cochlear Hair Cells |
| Alternative Antibody Names | Anti-Transmembrane Channel-Like Protein 1 antibodyAnti-Transmembrane Cochlear-Expressed Protein 1 antibodyAnti-TMC1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
