Anti-TLR8 antibody (849-1041) (STJ25872)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TLR8 (849-1041) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TLR8 |
| Gene ID | 51311 |
| Uniprot ID | TLR8_HUMAN |
| Immunogen Region | 849-1041 |
| Immunogen Sequence | HHLFYWDVWFIYNVCLAKVK GYRSLSTSQTFYDAYISYDT KDASVTDWVINELRYHLEES RDKNVLLCLEERDWDPGLAI IDNLMQSINQSKKTVFVLTK KYAKSWNFKTAFYLALQRLM DENMDVIIFILLEPVLQHSQ YLRLRQRICKSSILQWPDNP KAEGLFWQTLRNVVLTENDS RYNNMYVDSIKQY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 849-1041 of human TLR8 (NP_619542.1). |
Additional Info
| Tissue Specificity | Expressed in myeloid dendritic cells, monocytes, and monocyte-derived dendritic cells. |
| Post Translational Modifications | Ubiquitinated by RNF216.leading to degradation by the proteasome. Proteolytic processing occurs in monocytes and monocyte-derived macrophages by both furin-like proprotein convertase and cathepsins. The cleavage is necessary for dimer formation and subsequent activation. |
| Function | Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2. Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively. |
| Protein Name | Toll-Like Receptor 8Cd Antigen Cd288 |
| Database Links | Reactome: R-HSA-1679131Reactome: R-HSA-168181Reactome: R-HSA-9705671 |
| Cellular Localisation | Endosome MembraneSingle-Pass Type I Membrane ProteinEndosomal Localization Confers Distinctive Proteolytic Processing |
| Alternative Antibody Names | Anti-Toll-Like Receptor 8 antibodyAnti-Cd Antigen Cd288 antibodyAnti-TLR8 antibodyAnti-UNQ249 antibodyAnti-PRO286 antibody |
Information sourced from Uniprot.org