• Western blot analysis of lysates from Jurkat, 293, and HUVEC cells, using TLE4 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using TLE4 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-TLE4 antibody (161-210 aa) (STJ96036)

SKU:
STJ96036

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transducin-like enhancer protein 4 (161-210 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLE4
Gene ID: 7091
Uniprot ID: TLE4_HUMAN
Immunogen Region: 161-210 aa
Specificity: TLE4 Polyclonal Antibody detects endogenous levels of TLE4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human TLE4 at the amino acid range 161-210
Post Translational Modifications Phosphorylated. PAX5 binding increases phosphorylation. Ubiquitinated by XIAP/BIRC4.
Function Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression. Involved in transcriptional repression of GNRHR and enhances MSX1-mediated transcriptional repression of CGA/alpha-GSU.
Protein Name Transducin-Like Enhancer Protein 4
Grg-4
Groucho-Related Protein 4
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-2122947
Reactome: R-HSA-3769402
Reactome: R-HSA-4641265
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transducin-Like Enhancer Protein 4 antibody
Anti-Grg-4 antibody
Anti-Groucho-Related Protein 4 antibody
Anti-TLE4 antibody
Anti-GRG4 antibody
Anti-KIAA1261 antibody

Information sourced from Uniprot.org

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