Anti-TLE1 antibody [ZN253] (STJA0033400)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenHuman recombinant protein fragment of human TLE1 (NP_005068) produced in E.coli.
General Information
| Short Description | Mouse monoclonal anti-TLE1 for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ZN253 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 0.5-1.0 mg/mL |
| Purification | Affinity Chromatography |
| Dilution Range | IHC 1:50-1:100 |
| Formulation | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | TLE1 |
| Gene ID | 7088 |
| Uniprot ID | TLE1_HUMAN |
| Immunogen | Human recombinant protein fragment of human TLE1 (NP_005068) produced in E.coli. |
Additional Info
| Tissue Specificity | In all tissues examined, mostly in brain, liver and muscle. |
| Post Translational Modifications | Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1. Ubiquitinated by XIAP/BIRC4. |
| Function | Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG. |
| Protein Name | Transducin-Like Enhancer Protein 1E(Sp1 HomologEnhancer Of Split Groucho-Like Protein 1Esg1 |
| Database Links | Reactome: R-HSA-201722Reactome: R-HSA-2122947Reactome: R-HSA-3769402Reactome: R-HSA-4641265 |
| Cellular Localisation | NucleusNuclear And Chromatin-AssociatedDepending On Isoforms And Phosphorylation StatusHyperphosphorylation Decreases The Affinity For Nuclear Components |
| Alternative Antibody Names | Anti-Transducin-Like Enhancer Protein 1 antibodyAnti-E(Sp1 Homolog antibodyAnti-Enhancer Of Split Groucho-Like Protein 1 antibodyAnti-Esg1 antibodyAnti-TLE1 antibody |
Information sourced from Uniprot.org