![Anti-TLE1 antibody (150-250) [S9MR] (STJ11101889)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90804/361125/STJ11101889_1__54688.1713125908.jpg?c=1 "Anti-TLE1 antibody (150-250) [S9MR] (STJ11101889)")
![Anti-TLE1 antibody (150-250) [S9MR] (STJ11101889)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/90804/361125/STJ11101889_1__54688.1713125908.jpg?c=1 "Anti-TLE1 antibody (150-250) [S9MR] (STJ11101889)")
Anti-TLE1 antibody (150-250) [S9MR] (STJ11101889)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal TLE1 (150-250) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S9MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000 IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TLE1 |
| Gene ID | 7088 |
| Uniprot ID | TLE1_HUMAN |
| Immunogen Region | 150-250 |
| Immunogen Sequence | LQPPGIPPLGGSAGLLALSS ALSGQSHLAIKDDKKHHDAE HHRDREPGTSNSLLVPDSLR GTDKRRNGPEFSNDIKKRKV DDKDSSHYDSDGDKSDDNLV V |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human TLE1 (Q04724). |
Additional Info
| Tissue Specificity | In all tissues examined, mostly in brain, liver and muscle. |
| Post Translational Modifications | Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1. Ubiquitinated by XIAP/BIRC4. |
| Function | Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG. |
| Protein Name | Transducin-Like Enhancer Protein 1E(Sp1 HomologEnhancer Of Split Groucho-Like Protein 1Esg1 |
| Database Links | Reactome: R-HSA-201722Reactome: R-HSA-2122947Reactome: R-HSA-3769402Reactome: R-HSA-4641265 |
| Cellular Localisation | NucleusNuclear And Chromatin-AssociatedDepending On Isoforms And Phosphorylation StatusHyperphosphorylation Decreases The Affinity For Nuclear Components |
| Alternative Antibody Names | Anti-Transducin-Like Enhancer Protein 1 antibodyAnti-E(Sp1 Homolog antibodyAnti-Enhancer Of Split Groucho-Like Protein 1 antibodyAnti-Esg1 antibodyAnti-TLE1 antibody |
Information sourced from Uniprot.org