• Anti-TLE1 antibody (150-250) [S9MR] (STJ11101889)
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using TLE1 Rabbit monoclonal antibody (STJ11101889) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TLE1 Rabbit monoclonal antibody (STJ11101889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-TLE1 antibody (150-250) [S9MR] (STJ11101889)

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STJ11101889

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TLE1 (150-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000 IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLE1
Gene ID: 7088
Uniprot ID: TLE1_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human TLE1 (Q04724).
Immunogen Sequence: LQPPGIPPLGGSAGLLALSS ALSGQSHLAIKDDKKHHDAE HHRDREPGTSNSLLVPDSLR GTDKRRNGPEFSNDIKKRKV DDKDSSHYDSDGDKSDDNLV V
Tissue Specificity In all tissues examined, mostly in brain, liver and muscle.
Post Translational Modifications Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1. Ubiquitinated by XIAP/BIRC4.
Function Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
Protein Name Transducin-Like Enhancer Protein 1
E(Sp1 Homolog
Enhancer Of Split Groucho-Like Protein 1
Esg1
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-2122947
Reactome: R-HSA-3769402
Reactome: R-HSA-4641265
Cellular Localisation Nucleus
Nuclear And Chromatin-Associated
Depending On Isoforms And Phosphorylation Status
Hyperphosphorylation Decreases The Affinity For Nuclear Components
Alternative Antibody Names Anti-Transducin-Like Enhancer Protein 1 antibody
Anti-E(Sp1 Homolog antibody
Anti-Enhancer Of Split Groucho-Like Protein 1 antibody
Anti-Esg1 antibody
Anti-TLE1 antibody

Information sourced from Uniprot.org

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