• Western blot analysis of various lysates, using TKT rabbit polyclonal antibody (STJ115514) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using TKT antibody (STJ115514) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TKT antibody (250-350) (STJ115514)

SKU:
STJ115514

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TKT (250-350) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TKT
Gene ID: 7086
Uniprot ID: TKT_HUMAN
Immunogen Region: 250-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human TKT (NP_001055.1).
Immunogen Sequence: GVEDKESWHGKPLPKNMAEQ IIQEIYSQIQSKKKILATPP QEDAPSVDIANIRMPSLPSY KVGDKIATRKAYGQALAKLG HASDRIIALDGDTKNSTFSE I
Function Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.
Protein Name Transketolase
Tk
Database Links Reactome: R-HSA-163754
Reactome: R-HSA-71336
Reactome: R-HSA-9818028
Alternative Antibody Names Anti-Transketolase antibody
Anti-Tk antibody
Anti-TKT antibody

Information sourced from Uniprot.org

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