• Western blot analysis of extracts of various cell lines, using Thymidine Kinase 1 antibody (STJ27579) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using Thymidine Kinase 1 rabbit polyclonal antibody (STJ27579) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using Thymidine Kinase 1 rabbit polyclonal antibody (STJ27579) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using Thymidine Kinase 1 rabbit polyclonal antibody (STJ27579) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Thymidine Kinase 1 rabbit polyclonal antibody (STJ27579) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using Thymidine Kinase 1 rabbit polyclonal antibody (STJ27579) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TK1 antibody (15-194) (STJ27579)

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STJ27579

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TK1 (15-194) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TK1
Gene ID: 7083
Uniprot ID: KITH_HUMAN
Immunogen Region: 15-194
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 15-194 of human Thymidine Kinase 1 (NP_003249.3).
Immunogen Sequence: SKTRGQIQVILGPMFSGKST ELMRRVRRFQIAQYKCLVIK YAKDTRYSSSFCTHDRNTME ALPACLLRDVAQEALGVAVI GIDEGQFFPDIVEFCEAMAN AGKTVIVAALDGTFQRKPFG AILNLVPLAESVVKLTAVCM ECFREAAYTKRLGTEKEVEV IGGADKYHSVCRLCYFKKAS
Post Translational Modifications Phosphorylated on Ser-13 in mitosis. Phosphorylation of Ser-13 by CDK1 during mitosis reduces homotetramerization and catalytic efficiency when DNA replication is complete and intracellular TK1 is still present at a high level. Polyubiquitinated. Postmitosis, ubiquitination leads to proteasomal degradation. The KEN box sequence located at the C-terminal region targets for degradation by the anaphase promoting complex (APC/C) activated and rate-limited by FZR1.
Function Cell-cycle-regulated enzyme of importance in nucleotide metabolism. Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate. Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT).
Protein Name Thymidine Kinase - Cytosolic
Database Links Reactome: R-HSA-69205
Reactome: R-HSA-73614
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Thymidine Kinase - Cytosolic antibody
Anti-TK1 antibody

Information sourced from Uniprot.org

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