• Confocal imaging of paraffin-embedded Human kidney tissue using TIMM50 Rabbit monoclonal antibody (STJ11101436, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Mouse kidney tissue using TIMM50 Rabbit monoclonal antibody (STJ11101436, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of NIH/3T3 cells using TIMM50 Rabbit monoclonal antibody (STJ11101436, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of TIMM50 in paraffin-embedded rat kidney tissue using TIMM50 Rabbit monoclonal antibody (STJ11101436) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of TIMM50 in paraffin-embedded human liver tissue using TIMM50 Rabbit monoclonal antibody (STJ11101436) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of TIMM50 in paraffin-embedded mouse kidney tissue using TIMM50 Rabbit monoclonal antibody (STJ11101436) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using TIMM50 Rabbit monoclonal antibody (STJ11101436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using TIMM50 Rabbit monoclonal antibody (STJ11101436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TIMM50 antibody (1-100) [S6MR] (STJ11101436)

SKU:
STJ11101436

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TIMM50 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMM50
Gene ID: 92609
Uniprot ID: TIM50_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TIMM50 (Q3ZCQ8).
Immunogen Sequence: MAASAAVFSRLRSGLRLGSR GLCTRLATPPRRAPDQAAEI GSRGSTKAQGPQQQPGSEGP SYAKKVALWLAGLLGAGGTV SVVYIFGNNPVDENGAKIPD
Tissue Specificity Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
Function Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro.however such activity may not be relevant in vivo. Isoform 2: May participate in the release of snRNPs and SMN from the Cajal body.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim50
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Isoform 2: Nucleus Speckle
Nuclear And Enriched In Speckles With Snrnps
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim50 antibody
Anti-TIMM50 antibody
Anti-TIM50 antibody
Anti-PRO1512 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance