• Immunofluorescence analysis of U-2 OS cells using TIMM44 Rabbit polyclonal antibody (STJ119562) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using TIMM44 Rabbit polyclonal antibody (STJ119562) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using TIMM44 Rabbit polyclonal antibody (STJ119562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TIMM44 antibody (153-452) (STJ119562)

SKU:
STJ119562

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIMM44 (153-452) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMM44
Gene ID: 10469
Uniprot ID: TIM44_HUMAN
Immunogen Region: 153-452
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 153-452 of human TIMM44 (NP_006342.2).
Immunogen Sequence: AKTAKQSAESVSKGGEKLGR TAAFRALSQGVESVKKEIDD SVLGQTGPYRRPQRLRKRTE FAGDKFKEEKVFEPNEEALG VVLHKDSKWYQQWKDFKENN VVFNRFFEMKMKYDESDNAF IRASRALTDKVTDLLGGLFS KTEMSEVLTEILRVDPAFDK DRFLKQCENDIIPNVLEAMI SGELDILKDWCYEATYSQLA HPIQQAKALGLQFHSRILDI DNVDLAMGKMMEQGPVLII
Function Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim44
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Mitochondrion Matrix
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim44 antibody
Anti-TIMM44 antibody
Anti-MIMT44 antibody
Anti-TIM44 antibody

Information sourced from Uniprot.org

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