• STJ73468 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ73468 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0. 4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-TIAM1 antibody (C-Term) (STJ73468)

SKU:
STJ73468-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-TIAM1 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TIAM1
Gene ID: 7074
Uniprot ID: TIAM1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_003244; NP_001340613.1; NP_001340614.1; NP_001340615.1; NP_001340616.1
Immunogen Sequence: EDFAPSRKLNTEI
Post Translational Modifications Ubiquitinated. Undergoes 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 by a CUL3(KBTBD6/7) E3 ubiquitin ligase complex composed of CUL3, RBX1, KBTBD6 and KBTBD7. 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 triggers proteasomal degradation. Ubiquitination at Lys-1404 and Lys-1420 by CUL3(KBTBD6/7) also requires the membrane-associated protein GABARAP and may therefore be spatially restricted within the cell.
Function Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration.
Protein Name Rho Guanine Nucleotide Exchange Factor Tiam1
T-Lymphoma Invasion And Metastasis-Inducing Protein 1
Tiam-1
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-3928662
Reactome: R-HSA-3928665
Reactome: R-HSA-416482
Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Reactome: R-HSA-9032845
Cellular Localisation Cell Junction
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Detected At The Boundary Between Cells With Actin-Rich Protrusions
Presence Of Krit1
Cdh5 And Rap1b Is Required For Its Localization To The Cell Junction
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor Tiam1 antibody
Anti-T-Lymphoma Invasion And Metastasis-Inducing Protein 1 antibody
Anti-Tiam-1 antibody
Anti-TIAM1 antibody

Information sourced from Uniprot.org

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