Anti-TIAL1 & TIA antibody (Internal) (STJ72117)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-TIAL1 & TIA (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 45kDa band observed in lysates of cell line K562 (calculated MW of 43.4kDa according to NP_001029097.1). Recommended concentration: 1-3µg/ml.IHC: In paraffin embedded Human |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | TIAL1 |
| Gene ID | 7073 |
| Uniprot ID | TIAR_HUMAN |
| Accession Number | NP_003243.1; NP_001029097.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | SPEITTEDIKSAFAP |
| Specificity | This antibody is expected to recognize both isoforms (NP_003243.1; NP_001029097.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA. |
| Function | RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation. Shows a preference for uridine-rich RNAs. Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA. Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1. Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. |
| Protein Name | Nucleolysin TiarTia-1-Related Protein |
| Database Links | Reactome: R-HSA-6803529 |
| Cellular Localisation | NucleusCytoplasmCytolytic GranuleStress GranuleNuclear Import Seems To Be Coupled To Rna Polymerase Ii Transcription And May Be Dependent On Rna-BindingAccumulates In Cytoplasmic Stress Granules (Sg) Following Cellular Damage |
| Alternative Antibody Names | Anti-Nucleolysin Tiar antibodyAnti-Tia-1-Related Protein antibodyAnti-TIAL1 antibody |
Information sourced from Uniprot.org