• Immunohistochemistry analysis of TIAL1 in paraffin-embedded mouse spleen using TIAL1 Rabbit polyclonal antibody (STJ27869) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TIAL1 in paraffin-embedded human appendix using TIAL1 Rabbit polyclonal antibody (STJ27869) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TIAL1 in paraffin-embedded rat lung using TIAL1 Rabbit polyclonal antibody (STJ27869) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TIAL1 Rabbit polyclonal antibody (STJ27869) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TIAL1 antibody (266-375) (STJ27869)

SKU:
STJ27869

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIAL1 (266-375) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIAL1
Gene ID: 7073
Uniprot ID: TIAR_HUMAN
Immunogen Region: 266-375
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 266-375 of human TIAL1 (NP_003243.1).
Immunogen Sequence: EGHVVKCYWGKESPDMTKNF QQVDYSQWGQWSQVYGNPQQ YGQYMANGWQVPPYGVYGQP WNQQGFGVDQSPSAAWMGGF GAQPPQGQAPPPVIPPPNQA GYGMASYQTQ
Tissue Specificity Expressed in brain, heart, kidney, lung and skeletal muscle.
Post Translational Modifications Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA.
Function RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation. Shows a preference for uridine-rich RNAs. Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA. Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1. Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
Protein Name Nucleolysin Tiar
Tia-1-Related Protein
Database Links Reactome: R-HSA-6803529
Cellular Localisation Nucleus
Cytoplasm
Cytolytic Granule
Stress Granule
Nuclear Import Seems To Be Coupled To Rna Polymerase Ii Transcription And May Be Dependent On Rna-Binding
Accumulates In Cytoplasmic Stress Granules (Sg) Following Cellular Damage
Alternative Antibody Names Anti-Nucleolysin Tiar antibody
Anti-Tia-1-Related Protein antibody
Anti-TIAL1 antibody

Information sourced from Uniprot.org

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