Anti-THOC2 antibody (1-270) (STJ11100288)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal THOC2 (1-270) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | THOC2 |
| Gene ID | 57187 |
| Uniprot ID | THOC2_HUMAN |
| Immunogen Region | 1-270 |
| Immunogen Sequence | MAAAAVVVPAEWIKNWEKSG RGEFLHLCRILSENKSHDSS TYRDFQQALYELSYHVIKGN LKHEQASNVLSDISEFREDM PSILADVFCILDIETNCLEE KSKRDYFTQLVLACLYLVSD TVLKERLDPETLESLGLIKQ SQQFNQKSVKIKTKLFYKQQ KFNLLREENEGYAKLIAELG QDLSGSITSDLILENIKSLI GCFNLDPNRVLDVILEVFEC RPEHDDFFISLLESYMSMC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human THOC2 (NP_001075019.1). |
Additional Info
| Tissue Specificity | Expressed in the hippocampus and the cerebral cortex. |
| Function | Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. Required for efficient export of polyadenylated RNA and spliced mRNA. The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B.in the complex THOC2 is the only component that directly interacts with DDX39B. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway. Required for NXF1 localization to the nuclear rim. THOC2 (and probably the THO complex) is involved in releasing mRNA from nuclear speckle domains. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. |
| Protein Name | Tho Complex Subunit 2Tho2Htrex120 |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72187Reactome: R-HSA-73856 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasm |
| Alternative Antibody Names | Anti-Tho Complex Subunit 2 antibodyAnti-Tho2 antibodyAnti-Htrex120 antibodyAnti-THOC2 antibodyAnti-CXorf3 antibody |
Information sourced from Uniprot.org