• Western blot analysis of extracts of various cell lines, using Nuclear Matrix Protein p84 (THOC1) (THOC1) rabbit monoclonal antibody (STJ11102469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using Nuclear Matrix Protein p84 (THOC1) (THOC1) Rabbit monoclonal antibody (STJ11102469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-THOC1 antibody (350-450) [S9MR] (STJ11102469)

SKU:
STJ11102469

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Nuclear Matrix Protein p84 (350-450) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: THOC1
Gene ID: 9984
Uniprot ID: THOC1_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Nuclear Matrix Protein p84 (THOC1) (NP_005122.2).
Immunogen Sequence: SLWIEDTTKSVYQLLSENPP DGERFSKMVEHILNTEENWN SWKNEGCPSFVKERTSDTKP TRIIRKRTAPEDFLGKGPTK KILMGNEELTRLWNLCPDNM E
Tissue Specificity Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
Post Translational Modifications Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass. Polyubiquitinated, leading to proteasomal degradation.probably involves NEDD4.
Function Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation. May play a role in hair cell formation, hence may be involved in hearing. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
Protein Name Tho Complex Subunit 1
Tho1
Nuclear Matrix Protein P84
P84n5
Htrex84
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72187
Reactome: R-HSA-73856
Cellular Localisation Isoform 1: Nucleus Speckle
Nucleus
Nucleoplasm
Nucleus Matrix
Cytoplasm
Can Shuttle Between The Nucleus And Cytoplasm
Nuclear Localization Is Required For Induction Of Apoptotic Cell Death
Translocates To The Cytoplasm During The Early Phase Of Apoptosis Execution
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Tho Complex Subunit 1 antibody
Anti-Tho1 antibody
Anti-Nuclear Matrix Protein P84 antibody
Anti-P84n5 antibody
Anti-Htrex84 antibody
Anti-THOC1 antibody
Anti-HPR1 antibody

Information sourced from Uniprot.org

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