• Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-TH antibody (STJ99604)

SKU:
STJ99604

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tyrosine 3-monooxygenase is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:10000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TH
Gene ID: 7054
Uniprot ID: TY3H_HUMAN
Specificity: This antibody detects endogenous levels of Tyrosine Hydroxylase.
Immunogen: Synthesized peptide derived from the human Tyrosine Hydroxylase Polyclonal
Post Translational Modifications Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities. Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity. Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines. Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway. Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network. Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG.this interaction may influence the phosphorylation and dephosphorylation of other sites. Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity.
Function Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity. Positively regulates the regression of retinal hyaloid vessels during postnatal development. Isoform 5: Lacks catalytic activity. Isoform 6: Lacks catalytic activity.
Protein Name Tyrosine 3-Monooxygenase
Tyrosine 3-Hydroxylase
Th
Database Links Reactome: R-HSA-209905
Cellular Localisation Cytoplasm
Perinuclear Region
Nucleus
Cell Projection
Axon
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle
When Phosphorylated At Ser-19 Shows A Nuclear Distribution And When Phosphorylated At Ser-31 As Well At Ser-40 Shows A Cytosolic Distribution
Expressed In Dopaminergic Axons And Axon Terminals
Alternative Antibody Names Anti-Tyrosine 3-Monooxygenase antibody
Anti-Tyrosine 3-Hydroxylase antibody
Anti-Th antibody
Anti-TH antibody
Anti-TYH antibody

Information sourced from Uniprot.org

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