Anti-TH antibody (1-100) [S4MR] (STJ11102244)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Tyrosine Hydroxylase (1-100) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TH |
| Gene ID | 7054 |
| Uniprot ID | TY3H_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MPTPDATTPQAKGFRRAVSE LDAKQAEAIMVRGQGAPGPS LTGSPWPGTAAPAASYTPTP RSPRFIGRRQSLIEDARKER EAAVAAAAAAVPSEPGDPLE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Tyrosine Hydroxylase (P07101). |
Additional Info
| Tissue Specificity | Mainly expressed in the brain and adrenal glands. |
| Post Translational Modifications | Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities. Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity. Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines. Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway. Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network. Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG.this interaction may influence the phosphorylation and dephosphorylation of other sites. Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity. |
| Function | Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity. Positively regulates the regression of retinal hyaloid vessels during postnatal development. Isoform 5: Lacks catalytic activity. Isoform 6: Lacks catalytic activity. |
| Protein Name | Tyrosine 3-MonooxygenaseTyrosine 3-HydroxylaseTh |
| Database Links | Reactome: R-HSA-209905 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleusCell ProjectionAxonCytoplasmic VesicleSecretory VesicleSynaptic VesicleWhen Phosphorylated At Ser-19 Shows A Nuclear Distribution And When Phosphorylated At Ser-31 As Well At Ser-40 Shows A Cytosolic DistributionExpressed In Dopaminergic Axons And Axon Terminals |
| Alternative Antibody Names | Anti-Tyrosine 3-Monooxygenase antibodyAnti-Tyrosine 3-Hydroxylase antibodyAnti-Th antibodyAnti-TH antibodyAnti-TYH antibody |
Information sourced from Uniprot.org