• Western blot analysis of extracts of various cell lines, using TGF beta induced (TGFBI) antibody (STJ25831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using TGF beta induced (TGFBI) antibody (STJ25831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using TGF beta induced (TGFBI) rabbit polyclonal antibody (STJ25831) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using TGF beta induced (TGFBI) rabbit polyclonal antibody (STJ25831) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using TGF beta induced (TGFBI) rabbit polyclonal antibody (STJ25831) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using TGF beta induced (TGFBI) rabbit polyclonal antibody (STJ25831) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Y79 cells using TGF beta induced (TGFBI) rabbit polyclonal antibody (STJ25831) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-TGFBI antibody (550-650) (STJ25831)

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STJ25831

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TGFBI (550-650) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TGFBI
Gene ID: 7045
Uniprot ID: BGH3_HUMAN
Immunogen Region: 550-650
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human TGF beta induced (TGFBI) (NP_000349.1).
Immunogen Sequence: LPPRERSRLLGDAKELANIL KYHIGDEILVSGGIGALVRL KSLQGDKLEVSLKNNVVSVN KEPVAEPDIMATNGVVHVIT NVLQPPANRPQERGDELADS A
Tissue Specificity Highly expressed in the corneal epithelium. Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post Translational Modifications Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.these residues may be required for binding to calcium. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues. The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain.
Function Plays a role in cell adhesion. May play a role in cell-collagen interactions.
Protein Name Transforming Growth Factor-Beta-Induced Protein Ig-H3
Beta Ig-H3
Kerato-Epithelin
Rgd-Containing Collagen-Associated Protein
Rgd-Cap
Database Links Reactome: R-HSA-977225
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
May Be Associated Both With Microfibrils And With The Cell Surface
Alternative Antibody Names Anti-Transforming Growth Factor-Beta-Induced Protein Ig-H3 antibody
Anti-Beta Ig-H3 antibody
Anti-Kerato-Epithelin antibody
Anti-Rgd-Containing Collagen-Associated Protein antibody
Anti-Rgd-Cap antibody
Anti-TGFBI antibody
Anti-BIGH3 antibody

Information sourced from Uniprot.org

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