Anti-TGFB2 antibody (20-442) (STJ25830)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TGFB2 (20-442) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TGFB2 |
| Gene ID | 7042 |
| Uniprot ID | TGFB2_HUMAN |
| Immunogen Region | 20-442 |
| Immunogen Sequence | SLSTCSTLDMDQFMRKRIEA IRGQILSKLKLTSPPEDYPE PEEVPPEVISIYNSTRDLLQ EKASRRAAACERERSDEEYY AKEVYKIDMPPFFPSENAIP PTFYRPYFRIVRFDVSAMEK NASNLVKAEFRVFRLQNPKA RVPEQRIELYQILKSKDLTS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human TGFB2 (NP_001129071.1). |
Additional Info
| Post Translational Modifications | Transforming growth factor beta-2 proprotein: The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-2 (TGF-beta-2) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-2 inactive. |
| Function | Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. |
| Protein Name | Transforming Growth Factor Beta-2 ProproteinCeterminGlioblastoma-Derived T-Cell Suppressor FactorG-Tsf Cleaved Into - Latency-Associated PeptideLap - Transforming Growth Factor Beta-2Tgf-Beta-2 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-2129379Reactome: R-HSA-2173789Reactome: R-HSA-3000178Reactome: R-HSA-9839389 |
| Cellular Localisation | Latency-Associated Peptide: SecretedExtracellular SpaceExtracellular MatrixTransforming Growth Factor Beta-2: Secreted |
| Alternative Antibody Names | Anti-Transforming Growth Factor Beta-2 Proprotein antibodyAnti-Cetermin antibodyAnti-Glioblastoma-Derived T-Cell Suppressor Factor antibodyAnti-G-Tsf Cleaved Into - Latency-Associated Peptide antibodyAnti-Lap - Transforming Growth Factor Beta-2 antibodyAnti-Tgf-Beta-2 antibodyAnti-TGFB2 antibody |
Information sourced from Uniprot.org