• Immunohistochemistry analysis of DP1/TFDP1 in paraffin-embedded human lung using DP1/TFDP1 Rabbit polyclonal antibody (STJ27375) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DP1/TFDP1 in paraffin-embedded human colon damage using DP1/TFDP1 Rabbit polyclonal antibody (STJ27375) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using DP1/TFDP1 Rabbit polyclonal antibody (STJ27375) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TFDP1 antibody (1-410) (STJ27375)

SKU:
STJ27375

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TFDP1 (1-410) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TFDP1
Gene ID: 7027
Uniprot ID: TFDP1_HUMAN
Immunogen Region: 1-410
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human DP1/TFDP1 (NP_009042.1).
Immunogen Sequence: MAKDAGLIEANGELKVFIDQ NLSPGKGVVSLVAVHPSTVN PLGKQLLPKTFGQSNVNIAQ QVVIGTPQRPAASNTLVVGS PHTPSTHFASQNQPSDSSPW SAGKRNRKGEKNGKGLRHFS MKVCEKVQRKGTTSYNEVAD ELVAEFSAADNHILPNESAY DQKNIRRRVYDALNVLMAMN IISKEKKEIKWIGLPTNSAQ ECQNLEVERQRRLERIKQKQ SQLQELILQQIAFKNLVQR
Tissue Specificity Highest levels in muscle. Also expressed in brain, placenta, liver and kidney. Lower levels in lung and pancreas. Not detected in heart.
Post Translational Modifications Phosphorylation by E2F1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation. Ubiquitinated by the BCR(KBTBD5) complex, leading to its subsequent degradation.
Function Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTCCGCGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters.
Protein Name Transcription Factor Dp-1
Drtf1-Polypeptide 1
Drtf1
E2f Dimerization Partner 1
Database Links Reactome: R-HSA-111448
Reactome: R-HSA-113501
Reactome: R-HSA-1362277
Reactome: R-HSA-1362300
Reactome: R-HSA-139915
Reactome: R-HSA-1538133
Reactome: R-HSA-1912408
Reactome: R-HSA-2173796
Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-6804114
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69656
Reactome: R-HSA-8953750
Reactome: R-HSA-9616222
Reactome: R-HSA-9661069
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Cytoplasm And Nucleus And Translocates Into The Nuclear Compartment Upon Heterodimerization With E2f1
Alternative Antibody Names Anti-Transcription Factor Dp-1 antibody
Anti-Drtf1-Polypeptide 1 antibody
Anti-Drtf1 antibody
Anti-E2f Dimerization Partner 1 antibody
Anti-TFDP1 antibody
Anti-DP1 antibody

Information sourced from Uniprot.org

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