• Western blot analysis of extracts of various cell lines, using Activator protein 2 (AP-2/TFAP2A) antibody (STJ11104502) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TFAP2A antibody (100-200) (STJ11104502)

SKU:
STJ11104502

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transcription Factor Ap-2-Alpha (100-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TFAP2A
Gene ID: 7020
Uniprot ID: AP2A_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Activator protein 2 (AP-2/TFAP2A) (NP_003211.1).
Immunogen Sequence: RQSQESGLLHTHRGLPHQLS GLDPRRDYRRHEDLLHGPHA LSSGLGDLSIHSLPHAIEEV PHVEDPGINIPDQTVIKKGP VSLSKSNSNAVSAIPINKDN L
Post Translational Modifications Sumoylated on Lys-10.which inhibits transcriptional activity.
Function Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Protein Name Transcription Factor Ap-2-Alpha
Ap2-Alpha
Ap-2 Transcription Factor
Activating Enhancer-Binding Protein 2-Alpha
Activator Protein 2
Ap-2
Database Links Reactome: R-HSA-3232118 P05549-1
Reactome: R-HSA-8864260
Reactome: R-HSA-8866904
Reactome: R-HSA-8866906
Reactome: R-HSA-8866907
Reactome: R-HSA-8866910
Reactome: R-HSA-8866911
Reactome: R-HSA-8869496
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Ap-2-Alpha antibody
Anti-Ap2-Alpha antibody
Anti-Ap-2 Transcription Factor antibody
Anti-Activating Enhancer-Binding Protein 2-Alpha antibody
Anti-Activator Protein 2 antibody
Anti-Ap-2 antibody
Anti-TFAP2A antibody
Anti-AP2TF antibody
Anti-TFAP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance